Pouring LB Agar Plates - Addgene

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About Search by Sequence

Search by Sequence performs a nucleotide-nucleotide or protein-translated nucleotide BLAST search against Addgene’s plasmid sequence database. BLAST returns plasmids with similarity to the query sequence.
Results are sorted by E-value, a statistic from BLAST that describes the significance of a match. Lower values are considered better matches.
FASTA headers and numbers at the beginning of each line will be removed. The query should only contain DNA characters.

Tips for Success

Enter a distinct sequence that is an important, differentiating feature. For example, the coding region of a gene, instead of the plasmid origin of replication.
Inspect the percent identity, query coverage, and alignment details to determine if a result match is satisfactory. Visit the corresponding plasmid webpage to view additional details about a matching plasmid.
If no results are returned:
- Try a different isoform or region of the desired sequence.
- Choose a different BLAST database. Try the general “All Addgene Plasmids” (default selection), instead of a specific database, such as “Plant Expression Plasmids”
- Try selecting a different BLAST algorithm:
  - megablast: Designed for comparing sequences within the same, or closely related, species. Default selection.
  - blastn: Designed for comparing sequences from different species. May return additional results, if exact species match is not required.
  - blastn-short: Optimized for searching with shorter sequences (