AsuHPI {HphI} - Vivantis Technologies Sdn Bhd

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Home Restriction Endonucleases Conventional Restriction Endonucleases AsuHPI {HphI}

AsuHPI {HphI}

Concentration2-5u/μl5'…GGTGA(N)8↓…3' 3'…CCACT(N)7↑…5'

Reaction Conditions 1X Buffer V330mM Tris-HCl (pH7.5 at 30°C), 10mM MgCl2, 100mM NaCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer10mM Tris-HCl (pH 7.5), 250mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation65°C for 20 minutes.

Ligation / Recutting Assay After 3-fold overdigestion with AsuHPI, 30% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AsuHPI for 16 hours at 37°C.Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)*Blocked by overlapping dam- methylation (GmATC): GGTGATC

Ordering Information

Catalog No Description Pack Size
RE1134 AsuHPI {HphI} 100u

 

DownloadsManualAsuHPI {HphI}

DownloadsMSDSAsuHPI {HphI}

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