Thermo Scientific™ HphI (10 U/µL)

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Thermo Scientific™ HphI (10 U/μL) Product Code. 10679900 Shop All Thermo Scientific Products Click to view available options Quantity: 1,500 units 300 units Unit Size: 1500 units 300 units This item is not returnable. View return policy

Product Code. 10679900

Brand: Thermo Scientific™ ER1101

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Description

The HphI restriction enzyme recognizes GGTGA(8/7)^ sites and cuts best at 37°C in B buffer (Isoschizomers: AsuHPI).

Lambda DNA (dam- digested with HphI, 1.4% agarose, 168 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...G G T G A (N)8▵...3'

3'...C C A C T (N)7▵...5'

Conditions for 100% Activity:

• 1X Buffer B: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer:

• HphI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

• Dam: may overlap —blocked
• Dcm: may overlap —no effect
• CpG: may overlap —no effect
• EcoKI: never overlaps —no effect
• EcoBI: may overlap —blocked

Note:

Assayed using lambda DNA (dam) (#SD0021). HphI is blocked by overlappingdam methylation. To avoiddam methylation, use adam-, dcm strain such as GM2163 (#M0099). High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.

Specifications

Specifications

Concentration	10 U/μL
Methylation Sensitivity	Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme	HphI
Compatible Buffer	10X Buffer B
Sensitive to Heat Inactivation	Yes
Optimal Reaction Temperature	37°C
Type IIS RE	Yes
Quantity	300 units
Product Type	Restriction Enzyme
Research Category	Scarless Cloning

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For Research Use Only. Not for use in diagnostic procedures.

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