FlyBase Experimental Tool Report: PhiC31:int

• Tools
  • Tools Overview & Help
  • Query by symbols/IDs
    • Batch Download
    • Sequence Downloader
    • ID Validator
    • Feature Mapper
  • Search/Browse Portals
    • QuickSearch
    • Vocabularies
    • QueryBuilder
    • CytoSearch
    • Sequenced Species
    • Interactions Browser
    • ImageBrowse
  • Genomics Tools
    • BLAST
    • Fly BLAST @ Alliance
    • JBrowse
    • CytoSearch
    • Feature Mapper
    • Chromosome Maps
    • Synteny Table
    • Coordinates Converter
    • Sequence Downloader
    • modENCODE RNA-Seq
      • Overview
      • RNA-Seq Similarity
      • RNA-Seq Profile
      • RNA-Seq By Region
      • JBrowse
  • Submit Data
    • Fast-Track Your Paper
    • Submit Personal Communication
    • Feedback on Gene Snapshots
• Downloads
  • Overview
  • Current Release
  • Archived Data
  • Map Conversion
  • Releases (FTP)
• Links
  • External Resources
  • Model Organisms (MODs)
    • Alliance of Genome Resources
    • BeeBase
    • DictyBase
    • EcoCyc
    • Gramene
    • MGI
    • PomBase
    • Pseudobase
    • RGD
    • SGD
    • TAIR
    • VectorBase
    • WormBase
    • Xenbase
    • ZFIN
  • Stock Collections
    • Bloomington Drosophila Stock Center (BDSC)
    • FlyORF
    • Korea Drosophila Resource Center (KDRC)
    • Kyoto Stock Center
    • NIG-FLY
    • Tsinghua Fly Center (THFC)
    • Vienna Drosophila Resource Center (VDRC)
  • BDGP
  • DGRC
  • DRSC/TRiP
  • DIS by issue
  • FlyBook
  • FlyExpress
  • Interactive Fly
  • Virtual Fly Brain
  • FlyCyc
• Community
  • Fast-Track Your Paper
  • FlyBase Community Advisory Group
  • Fly Lab List
  • Recent Papers With Technical Advances
  • Gene Snapshots
  • FlyBase Forum
  • Newsletter
  • FlySlack Community
  • Mastodon
  • Bluesky
  • X (formerly Twitter)
  • Fly Board
  • NIH Funding
• About
  • Release Notes
  • New In This Release
  • Release Schedule
  • FlyBase Positions
  • Citing FlyBase
  • FlyBase Licensing
  • FlyBase Consortium
  • FlyBase Publications
  • FlyBase Presentations
  • Grants Supporting FlyBase
• Help
  • Contact FlyBase
  • FlyBase Wiki:
  • Help Index
  • Tool help
  • Report help
  • FlyBase FAQ
  • Info for Authors
    • Author guidelines
    • Citing FlyBase
    • Fast-Track Your Paper Help
    • Gene Snapshots
    • Personal communications
  • Linking to/from FlyBase
  • Nomenclature
  • Curation documentation
    • Controlled Vocabularies in FlyBase
    • Gene Model Annotation
    • Gene Ontology (GO) Annotation
    • Nontraditional alleles
    • Vectors & Constructs
  • New to Flies?
  • Video Tutorials
  • Programmatic Access to FlyBase: APIs, etc.

FlyBase in AWS Open Data FlyBase NIH Grant Terminated Previous Next FB2025_05 , released December 11, 2025 Open Close General Information Symbol phiC31:int FlyBase ID FBto0000360 Name phiC31 integraseDescription Description

The phiC31:int entry in FlyBase represents the integrase encoded by the phiC31 bacteriophage. phiC31:int mediates recombination between two non-identical target sites, attP and attB, each of which contains a short integration core flanked by imperfect inverted repeats. Exchange between the attP/attB pair results in the formation of hybrid sites (attL and attR) that are no longer a substrate of the phiC31:int integrase, and thus the exchange reaction driven by phiC31:int is unidirectional (reviewed in PMID:21195181, FBrf0231034). The recombination event catalyzed by phiC31:int results in genetic modification, the nature of which is influenced by the relative orientation (direct or inverted), and location of the two target sites. The types of possible modification include deletion of DNA, generation of chromosomal rearrangements, integration of DNA into the genome, and replacement of genetic material with that from a donor plasmid using recombination-mediated cassette exchange (RMCE) (reviewed in FBrf0231034). For integration of DNA into the genome, the 'docking site' in the genome typically contains the attP site and the donor plasmid containing the sequence to be integrated contains the attB site, since this directionality gives higher integration rates compared to the alternative combination where the attB site is integrated into the genome (PMID:11359900, PMID:12563279).

Uses

• integrase

External Crossreferences and Linkouts Related experimental toolsCommon gene of origin (4) ToolUsesphiC31:Dint(HEE)genome engineering toolphiC31:Dint(HEG)genome engineering toolphiC31:Dint(KEE)genome engineering toolphiC31:Dint(KEK)genome engineering toolCompatible tools (4) ToolUsesattBintegrase target siteattB-CCintegrase target siteattPintegrase target siteattP-CCintegrase target site Transgenic Constructs Encodes tool (9) Export to HitList Transgenic construct(s) Component allele Reg. region Encoded product / toolShow UsesTagged withShow Uses Also carries Show Uses Stocks

• M{vas-int.Dm}
• M{vas-int.Dm.RFP-.GFP-}

phiC31\intDm.vas

• vas

phiC31:intintegrase81

• P{nanos-phiC31\int.NLS}
• M{nanos-int.NLS}

phiC31\intnanos.Tag:NLS(SV40-largeT)

• nanos

phiC31:intintegraseTag:NLS(SV40-largeT)nuclear protein localization tag28

• M{vas-int.B}

phiC31\intvas.PB

• vas

phiC31:intintegrase11

• P{nSyb-IVS-phiC31}

phiC31\intnSyb.PIB

• nSyb

phiC31:intintegrase3

• P{20XUAS-IVS-phiC31}

phiC31\int20XUAS.cIBa

• UAS

phiC31:intintegrase2

• P{αTub84B-IVS-phiC31}

phiC31\intαTub84B.PIB

• αTub84B

phiC31:intintegrase1

• M{nanos-int.B}

phiC31\intnanos.PB

• nanos

phiC31:intintegrase

• M{vas-int.NLS}

phiC31\intvas.Tag:NLS(SV40-largeT)

• vas

phiC31:intintegraseTag:NLS(SV40-largeT)nuclear protein localization tag

• P{UAS-phiC31:int.O}

phiC31\intUAS.cOa

• UAS

phiC31:intintegrase Insertions ( 0 ) Synonyms and Secondary IDs (2) Reported As Symbol Synonym phiC31:int Name Synonyms phiC31 integrase Secondary FlyBase IDs References (5)

Report Sections

Open Close

• General Information
• Description
• Transgenic constructs
• Insertions
• Synonyms
• References