PhiC31 integrase can integrate a plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable.
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Nonviral transgene delivery. The phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31.
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phiC31 integrase is a site-specific recombinase from a bacteriophage that has become a useful tool in mammalian cells. The enzyme normally performs precise, ...
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The phiC31 bacteriophage uses a distinct integrase to catalyze directed recombination at sequence-specific sites in the Streptomyces genome (Smith, et al.
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The PhiC31 Integrase System is an non-viral, integrase-based gene expression system for low- or single-copy integrations up to large insert sizes.
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23 thg 2, 2010 · The large serine recombinase phiC31 from broad host range Streptomyces temperate phage, catalyzes the site-specific recombination of two ...
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The bacteriophage phiC31 integrase is a recombinase that mediates the sequence-specific recombination between two attachment sites, attB and attP. Because these ...
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25 thg 11, 2020 · phiC31 integrase is a phage-derived site-specific recombinase that has been used for genomic manipulations in E. coli (Merrick et al.
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12 thg 6, 2020 · The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific genomic integration in mammalian cells, ...
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14 thg 9, 2012 · phiC31 was used in Schizosaccharomyces pombe [26] and several experimental animal systems, including Xenopus laevis [27], [28] and Drosophila [ ...
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w+/total no. ... The lines injected were homozygous for both an attP site and a φ C31 integrase construct. G0 adults (male and females) obtained after injection ...
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phiC31 and other site-specific recombinases have been used to invert or delete DNA segments as both components and outputs in synthetic recorders and logic ...
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The phiC31:int entry in FlyBase represents the integrase encoded by the phiC31 bacteriophage. phiC31:int mediates recombination between two non-identical ...
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25 thg 2, 2018 · Gene targeting plays a pivotal role in creating modified stem cells, which are considered the mainstay in regenerative medicine. The phiC31 ...
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