FlyBase Reference Report: Dai Et Al., 2018, PLoS Genet. 14(9)

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FlyBase in AWS Open Data FlyBase NIH Grant Terminated Previous Next FB2025_05 , released December 11, 2025 Open Close Reference Citation Dai, J., Estrada, B., Jacobs, S., Sánchez-Sánchez, B.J., Tang, J., Ma, M., Magadán-Corpas, P., Pastor-Pareja, J.C., Martín-Bermudo, M.D. (2018). Dissection of Nidogen function in Drosophila reveals tissue-specific mechanisms of basement membrane assembly. PLoS Genet. 14(9): e1007483. FlyBase ID FBrf0240333 Publication Type Research paper Abstract Basement membranes (BMs) are thin sheet-like specialized extracellular matrices found at the basal surface of epithelia and endothelial tissues. They have been conserved across evolution and are required for proper tissue growth, organization, differentiation and maintenance. The major constituents of BMs are two independent networks of Laminin and Type IV Collagen in addition to the proteoglycan Perlecan and the glycoprotein Nidogen/entactin (Ndg). The ability of Ndg to bind in vitro Collagen IV and Laminin, both with key functions during embryogenesis, anticipated an essential role for Ndg in morphogenesis linking the Laminin and Collagen IV networks. This was supported by results from cultured embryonic tissue experiments. However, the fact that elimination of Ndg in C. elegans and mice did not affect survival strongly questioned this proposed linking role. Here, we have isolated mutations in the only Ndg gene present in Drosophila. We find that while, similar to C.elegans and mice, Ndg is not essential for overall organogenesis or viability, it is required for appropriate fertility. We also find, alike in mice, tissue-specific requirements of Ndg for proper assembly and maintenance of certain BMs, namely those of the adipose tissue and flight muscles. In addition, we have performed a thorough functional analysis of the different Ndg domains in vivo. Our results support an essential requirement of the G3 domain for Ndg function and unravel a new key role for the Rod domain in regulating Ndg incorporation into BMs. Furthermore, uncoupling of the Laminin and Collagen IV networks is clearly observed in the larval adipose tissue in the absence of Ndg, indeed supporting a linking role. In light of our findings, we propose that BM assembly and/or maintenance is tissue-specific, which could explain the diverse requirements of a ubiquitous conserved BM component like Nidogen. PubMed ID 30260959 PubMed Central ID PMC6177204 (PMC) (EuropePMC) DOI 10.1371/journal.pgen.1007483 Related Publication(s) Personal communication to FlyBase

Location data for Ndg deletions.Pastor J., 2019.2.13, Location data for Ndg deletions. [FBrf0241454]

Associated Information Comments Associated Files Other Information Secondary IDs Language of Publication English Additional Languages of Abstract Parent Publication Publication Type Journal Abbreviation PLoS Genet. Title PLoS Genetics Publication Year 2005- ISBN/ISSN 1553-7404 1553-7390 Data From Reference Aberrations (9) Export to HitList

• Df(2L)BSC172
• Df(2L)LanB1
• Df(2R)BSC281
• Df(2R)Ndg-1
• Df(2R)Ndg-15
• Df(2R)Ndg-49
• Df(2R)Ndg-72
• Df(2R)Ndg-111
• Df(2R)Ndg-118

Alleles (44) Export to HitList

• Avic\GFPRNAi.sfGFP.UAS
• Avic\GFPRNAi.UAS.V20.3
• Avic\GFPtrol-CPTI002049
• Avic\GFPvkg-G00454
• Col4a1GD12784
• Col4a1UAS.mRFP1
• LanA160
• LanA216
• LanAJF02908
• LanB1fTRG00681.sfGFP-TVPTBF
• LanB1GD13179
• LanB2HMC04076
• Ndg1
• Ndg2
• NdgΔG1.UAS.EGFP
• NdgΔG2.UAS.EGFP
• NdgΔG2Rod.UAS.EGFP
• NdgΔG3.UAS.EGFP
• NdgΔRod.UAS.EGFP
• NdgΔRod-G3.1
• NdgΔRod-G3.2
• NdgΔRod-G3.3
• NdgfTRG00638.sfGFP-TVPTBF
• NdgG1.UAS.EGFP
• NdgG1L.UAS.EGFP
• NdgG1LG2.UAS.EGFP
• NdgG2.UAS.EGFP
• NdgG2Rod.UAS.EGFP
• NdgG3.UAS.EGFP
• NdgHMJ24142
• NdgL.UAS.EGFP
• NdgRod.UAS.EGFP
• NdgRodG3.UAS.EGFP
• NdgUAS.EGFP
• Scer\GAL4Act.PU
• Scer\GAL4Cg.PA
• Scer\GAL4en-e16E
• Scer\GAL4Mef2.PR
• Scer\GAL4SPARC-MI00329-GAL4
• Scer\GAL80ts.αTub84B
• trolGD7744
• trolJF03376
• trolnull
• vkgNIG.16858R

Genes (15) Export to HitList

• Avic\GFP
• CG12909
• CG34222
• Col4a1
• LanA
• LanB1
• LanB2
• Ndg
• Obp46a
• RNaseZ
• Scer\GAL4
• Scer\GAL80
• SPARC
• trol
• vkg

Natural transposons (1) Export to HitList

• P-element

Insertions (7) Export to HitList

• Mi{ET1}NdgMB12298
• Mi{GT-GAL4}SPARCMI00329-GAL4
• P{CaryP}Msp300attP40
• P{en2.4-GAL4}e16E
• P{PTT-un}vkgG00454
• P{UAS-sfGFP.RNAi}attP40
• PBac{681.P.FSVS-1}trolCPTI002049

Experimental Tools (3) Export to HitList

• EGFP
• UASt
• UAS

Transgenic Constructs (30) Export to HitList

• P{Act-GAL4.U}
• P{Cg-GAL4.A}
• P{GAL4-Mef2.R}
• P{GD7744}
• P{GD12784}
• P{GD13179}
• P{NIG.16858R}
• P{TRiP.HMC04076}
• P{TRiP.HMJ24142}
• P{TRiP.JF02908}
• P{TRiP.JF03376}
• P{tubP-GAL80ts}
• P{UAS-Col4a1.RFP}
• P{UAS-Ndg.ΔG1.EGFP}
• P{UAS-Ndg.ΔG2.EGFP}
• P{UAS-Ndg.ΔG2Rod.EGFP}
• P{UAS-Ndg.ΔG3.EGFP}
• P{UAS-Ndg.ΔRod.EGFP}
• P{UAS-Ndg.FL.EGFP}
• P{UAS-Ndg.G1.EGFP}
• P{UAS-Ndg.G1L.EGFP}
• P{UAS-Ndg.G1LG2.EGFP}
• P{UAS-Ndg.G2.EGFP}
• P{UAS-Ndg.G2Rod.EGFP}
• P{UAS-Ndg.G3.EGFP}
• P{UAS-Ndg.L.EGFP}
• P{UAS-Ndg.Rod.EGFP}
• P{UAS-Ndg.RodG3.EGFP}
• P{UAS-sfGFP.RNAi}
• P{VALIUM20-EGFP.RNAi.3}

Transcripts (1) Export to HitList

• Scer\GAL4SPARC-MI00329-GAL4RA

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• Data From Reference