22 thg 2, 2018 · G4 ChIP-seq detects chromatin regions that form G4 DNA structures in a cell population. As this is an enrichment-based method, it can detect ...
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23 thg 6, 2021 · By G4-ChIP-seq/RNA-seq analysis on liposarcoma cells we confirmed that G4s in promoters are invariably associated with high transcription ...
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Here, we describe a detailed G4 ChIP-seq method that robustly enables the determination of G4 structure formation genome-wide in chromatin.
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The most common G4 ChIP-Seq protocol entails immunoprecipitation of formaldehyde-fixed and sheared chromatin with the phage-display derived monoclonal BG4 ... INTRODUCTION · MATERIALS AND METHODS · RESULTS · DISCUSSION
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23 thg 4, 2021 · In contrast to G4 ChIP-seq on fixed chromatin, the study mapped over 100,000 G4s and observed considerable G4 formation downstream of the TSS in ...
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7 thg 5, 2021 · G4 sequence motifs are prevalent at promoters in the human genome [2,3,4], and ChIP-sequencing using a G4 structure-specific antibody has ...
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26 thg 4, 2021 · G4 ChIP-seq is a method which couples formaldehyde-fixed G4 DNA immunoprecipitation using a G4-specific antibody (most commonly the ...
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16 thg 8, 2021 · Chromatin immunoprecipitation with an engineered G4 structure-specific antibody BG4 followed by high-throughput sequencing (G4 ChIP-seq) has ...
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G4-chromatin immunoprecipitation sequencing (G4-ChIP-seq) peaks show statistically significant colocalization with CTCF-bound sites [6].
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Error bars, s.d. (n = 3). (c) Enrichment of HaCaT G4 ChIP–seq peaks relative to what is expected by randomly shuffling G4 ChIP–seq peaks in OQs and the absolute ...
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12 thg 8, 2021 · Recently, new sequencing techniques were developed to map G4s in vitro (G4-seq) and G4s in vivo (G4 ChIP-seq) at few hundred base resolution ...
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1 thg 4, 2022 · ChIP-seq. CUT&Tag. DNA. DRIP-seq. DRIPc-seq. DSB. DNase. ESC. ERV. FriP. G4P. GMP. G4. HR. HRDC. HDAC. IAP. KAS-seq. Adenosine triphosphate.
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27 thg 2, 2022 · Through G4 ChIP-seq, the formation of G4s in the chromatins has been ... RNA G4-binding proteins mostly have intrinsically disordered ...
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All in-house sequencing data have been deposited in the NCBI GEO database under accession number GSE145090. Additional replicates for G4 ChIP-seq in K562 cells ...
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