Genome-wide Mapping Of Endogenous G-quadruplex DNA ...

Trang chủ » G4 Chip-seq » Genome-wide Mapping Of Endogenous G-quadruplex DNA ...

Có thể bạn quan tâm

Clipboard, Search History, and several other advanced features are temporarily unavailable. Skip to main page content Dot gov

The .gov means it’s official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation pubmed logo Search: Search Advanced Clipboard User Guide Save Email Send to
  • Clipboard
  • My Bibliography
  • Collections
  • Citation manager
Display options Display options Format Abstract PubMed PMID

Save citation to file

Format: Summary (text) PubMed PMID Abstract (text) CSV Create file Cancel

Email citation

Email address has not been verified. Go to My NCBI account settings to confirm your email and then refresh this page. To: Subject: Body: Format: Summary Summary (text) Abstract Abstract (text) MeSH and other data Send email Cancel

Add to Collections

  • Create a new collection
  • Add to an existing collection
Name your collection: Name must be less than 100 characters Choose a collection: Unable to load your collection due to an error Please try again Add Cancel

Add to My Bibliography

  • My Bibliography
Unable to load your delegates due to an error Please try again Add Cancel

Your saved search

Name of saved search: Search terms: Test search terms Would you like email updates of new search results? Saved Search Alert Radio Buttons
  • Yes
  • No
Email: (change) Frequency: Monthly Weekly Daily Which day? The first Sunday The first Monday The first Tuesday The first Wednesday The first Thursday The first Friday The first Saturday The first day The first weekday Which day? Sunday Monday Tuesday Wednesday Thursday Friday Saturday Report format: Summary Summary (text) Abstract Abstract (text) PubMed Send at most: 1 item 5 items 10 items 20 items 50 items 100 items 200 items Send even when there aren't any new results Optional text in email: Save Cancel

Create a file for external citation management software

Create file Cancel

Your RSS Feed

Name of RSS Feed: Number of items displayed: 5 10 15 20 50 100 Create RSS Cancel RSS Link Copy

Full text links

Nature Publishing Group full text link Nature Publishing Group Full text links

Actions

CiteCollectionsAdd to Collections
  • Create a new collection
  • Add to an existing collection
Name your collection: Name must be less than 100 characters Choose a collection: Unable to load your collection due to an errorPlease try again Add Cancel PermalinkPermalinkCopyDisplay options Display options Format AbstractPubMedPMID

Page navigation

  • Title & authors
  • Abstract
  • References
  • Publication types
  • MeSH terms
  • Substances
  • Grants and funding
  • LinkOut - more resources
Title & authors Abstract References Publication types MeSH terms Substances Grants and funding LinkOut - more resources Full text links CiteDisplay options Display options Format AbstractPubMedPMID

Abstract

G-rich DNA sequences can form four-stranded G-quadruplex (G4) secondary structures and are linked to fundamental biological processes such as transcription, replication and telomere maintenance. G4s are also implicated in promoting genome instability, cancer and other diseases. Here, we describe a detailed G4 ChIP-seq method that robustly enables the determination of G4 structure formation genome-wide in chromatin. This protocol adapts traditional ChIP-seq for the detection of DNA secondary structures through the use of a G4-structure-specific single-chain antibody with refinements in chromatin immunoprecipitation followed by high-throughput sequencing. This technology does not require expression of the G4 antibody in situ, enabling broad applicability to theoretically all chromatin sources. Beginning with chromatin isolation and antibody preparation, the entire protocol can be completed in <1 week, including basic computational analysis.

PubMed Disclaimer

References

    1. J Am Chem Soc. 2013 Apr 3;135(13):5017-28 - PubMed
    1. Cell. 2011 Dec 9;147(6):1408-19 - PubMed
    1. Nucleic Acids Res. 2014 Jan;42(2):860-9 - PubMed
    1. Nat Commun. 2013;4:1796 - PubMed
    1. Nat Rev Genet. 2012 Nov;13(11):770-80 - PubMed
Show all 49 references

Publication types

  • Research Support, Non-U.S. Gov't Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search

MeSH terms

  • Animals Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Cell Line Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Chromatin Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Chromatin Immunoprecipitation / methods* Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Chromosome Mapping / methods* Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Chromosome Structures / genetics Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Chromosome Structures / physiology Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • DNA Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • DNA Replication Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • G-Quadruplexes* Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Genome-Wide Association Study Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Genomic Instability Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Guanosine / analysis Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • High-Throughput Nucleotide Sequencing / methods Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Humans Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search

Substances

  • Chromatin Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • Guanosine Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search
  • DNA Actions
    • Search in PubMed
    • Search in MeSH
    • Add to Search

Grants and funding

  • 19836/CRUK_/Cancer Research UK/United Kingdom

LinkOut - more resources

  • Full Text Sources

    • Nature Publishing Group

  • Other Literature Sources

    • The Lens - Patent Citations Database
    • scite Smart Citations

  • Research Materials

    • Addgene Non-profit plasmid repository
Full text links [x] Nature Publishing Group full text link Nature Publishing Group [x] Cite Copy Download .nbib .nbib Format: AMA APA MLA NLM Send To
  • Clipboard
  • Email
  • Save
  • My Bibliography
  • Collections
  • Citation Manager
[x]

NCBI Literature Resources

MeSH PMC Bookshelf Disclaimer

The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.

Từ khóa » G4 Chip-seq