RAS(K117N) - NewEast Biosciences

Trang chủ » K117n » RAS(K117N) - NewEast Biosciences

Có thể bạn quan tâm

Skip to content

Email: [email protected]   |  Call: 6109452007

china-flag-transparent-20 Search $0.00 0 Cart
  • Home
  • Products
  • Icon-In-PNG-2 Targets
  • Services
  • Top Citations
  • Lab Supplies

About NewEast

NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.

We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.

Bioactive Transmembrane Proteins
  • Proteins Embedded in Synthetic NanodiscsNew
  • Virus-like particles (VLPs)
  • Membrane Nanoparticles (MNPs)
  • Extracellular Domain Proteins New
Antibodies for Transmembrane Proteins
  • Biosimilar Reference Antibodies
  • Rabbit Monoclonal Antibodies
  • Nanobodies for Membrane Proteins
Lipoproteins
  • Low-density Lipoprotein
  • High-density Lipoprotein

cAMP & cGMP

  • Antibodies for cAMP & cGMP
  • No Acetylation EIA Kits

GTPase

  • Pull-Down Activation Assay Kits
  • Antibodies for Active GTPase
  • Antibodies for Total G Proteins

Oncogene

  • Antibodies for Mutated Oncogene
  • Antibodies for Total Oncogene

Reagents

  • MW Markers
  • Loading Control
  • ADC Reagents
  • IgG Fluorescent Label
  • Payload Detection

Recombinat Proteins

  • Proteins for GTPase & Oncogene

GTP-bound GTPase

Arf6 Arl1 Arl3 Cdc42 Gα12 Gα13 Gα14 Gαi Gαo Gαq
Gαs Gαz Rab11 Rab12 Rab14 Rab17 Rab18 Rab25 Rab26 Rab28
Rab2A Rab3 Rab30 Rab31 Rab35 Rab38 Rab39 Rab41 Rab4A Rab5
Rab7 Rab8A Rab9A Ral Rac1 Ran Rap1 Ras Rheb RhoA Sar1

BioMarkers

Albumin Apolipoprotein BAG1 BMP3 Cystatin EYA2
FASCIN GAPDH GRB7 KIM1 MUC1 ORAIL
RAC-8A SEPTIN9 SLC7A5 SUMO Survivin TFF3
Vimentin Transferrin β-Actin β-Tubulin

Mutated Oncogene

ABL AKT ALK ATM ß-CATENIN BRAF BRCA1
BRCA2 CTCF CTNNB1 EGFR ERBB2 FBXW7 FGFR1
GNAQ HRAS IDH JAK2 KIT MEK1 NFE2L2
NOTCH1 PIK3CA PTEN RAS RET RHEB SMAD4
SMO STK11 TP53

cAMP & cGMP - No Acetylation

cAMP cGMP

Antibody Production

Protein Production

Citations on Top 50 Journals (Google Scholar)

  • Rank    Journal Name    
  • 1.     Nature
  • 7.     Nature Communications
  • 8.     Cell
  • 13.     Proceedings of the National Academy of Sciences
  • 18.     Nucleic Acids Research
  • 20.    Nature Medicine
  • 25.     ACS Nano
  • 29.     Scientific Reports
  • 33.     PLoS ONE
  • 34.     Science Advances
  • 37.     Nature Genetics
  • 41.     International Journal of Molecular Sciences
  • 49.     Frontiers in Immunology

Citations Ranked by Product  

  • Rank    Product    
  • 1.     26903 Rac1-GTP
  • 2.     26904 RhoA-GTP
  • 3.     26905 Cdc42-GTP
  • 4.     80203 cAMP EIA Kit – No Acetylation
  • 5.     80103 cGMP EIA Kit – No Acetylation
  • 6.     26901 Gαi-GTP
  • 7.     26039 BRAF(V600E)
  • 8.    26909 Ras-GTP
  • 9.     26911 Rab5-GTP
  • 10.     26163 IDH2(R172K)
  • 11.     26919 Rab11-GTP
  • 12.     26910 Reb-GTP
  • 13.     26036 Ras(G13D)

Images from Citations

Rac1-GTP IF/IHC # 01 Rac1-GTP IF/IHC #02
RhoA-GTP IF/IHC # 01 RhoA-GTP IF/IHC #02
Ras -GTP IF and IHC Ras -GTP IP
Cdc42-GTP IF/IHC # 01 Cdc42-GTP IF/IHC # 02
Gαi -GTP IF, IHC and IP Gα13 -GTP IF, IHC and IP
Gαs -GTP IF, IHC and IP Rheb -GTP IF, IHC and IP
Rab5 -GTP IF, IHC and IP Rap1-GTP IF, IHC and IP
Ral-GTP IF, IHC and IP Ran-GTP IF, IHC and IP
Sar1-GTP IF, IHC and IP Arf6-GTP IF, IHC and IP
Rab11-GTP IF, IHC and IP
Supplies from Corning, Thermo Scientific and CellTreat with 50% or more Discount
  • Cell Strainers
  • Centrifuge Tubes , Centrifuge Tubes with Filter or Clear Test Tube
  • Erlenmeyer Flask (250 mL, 500 mL, 1 L and 5 L)
  • Filters and Filtration
  • Microplates ( 6-well, 48-well and 96 well)
  • Pipette Tips
  • Serological Pipets
  • Storage Bottles or Growth Flasks
  • Syringes and Needles
Bioactive Transmembrane Proteins - For Drug Discovery
  • Synthetic NanodiscsNew - Target protein with sounding lipid biolayer embedded in novel synthetic Nanodisc
  • Extracellular Domain Proteins (ECD)New
  • Biosimilar Reference AntibodiesNew - Speed up your drug discovery

Reagents

  • IgG Fluorescent LabelNew - For Antibody Internalization Activity Monitoring

Product Categories

Download Files

DataSheet

MSDS

RAS(K117N)

Anti-RAS(K117N) Mouse Monoclonal Antibody
5/5

$515.00

Cat.#: 26468

Size: 100 μl

In Stock

RAS(K117N) quantity Add to cart Product Description Related Products Publications Product Description RAS(K117N) 
Cat. #:  26468
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti-RAS(K117N) Mouse Monoclonal Antibody
Background:   The K117N mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras K117N mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Immunogen:   A synthetic peptide from the internal region of Ras which includes the mutation of K117N, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes K117N mutant, but not wild type RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant RAS(K117N) and wild type proteins. Purified His-tagged RAS(K117N) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-RAS(K117N) monoclonal antibody (Cat. #26468). Immunofluorescence:   Immunofluorescence of cells expressing KRas proteins with anti-KRAS(K117N) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (WT) plasmid (left column) or pCDNA3-GFP-KRAS(K117N) plasmid (right column), then fixed and stained with anti-KRAS(K117N) monoclonal antibody (Cat. #26468).
Related Products

Related products

  • Placeholder

    Ras mAb

    $255.00 Add to cart
  • Placeholder

    Arl3-GTP

    $515.00 Add to cart
  • Placeholder

    Gαi-GTP

    $515.00 Add to cart
  • Placeholder

    Rab11-GTP

    $515.00 Add to cart
Publications
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136
Circulating Tumor Cell Separator (CTC Separation by Filtration)
Antibody Internalization Labeling Kit
Name Brand Lab Supplies
Biosimilar Reference Antibodies
Synthetic Nanodisc
No Acetylation cAMP/cGMP EIA Kits
  • Citations Ranked by Product
  • 1. 26903 Rac1-GTP
  • 2. 26904 RhoA-GTP
  • 3. 26905 Cdc42-GTP
  • 4. 80203 cAMP EIA Kit – No Acetylation
  • 5. 80103 cGMP EIA Kit – No Acetylation
  • 7. 26039 BRAF(V600E)
  • 6. 26901 Gαi-GTP
  • 8. 26909 Ras-GTP
  • 9. 26911 Rab5-GTP
  • 10. 26163 IDH2(R172K)
  • 11. 26919 Rab11-GTP
  • 12. 26910 Reb-GTP
  • 13. 26036 Ras(G13D)
Select by Alphabet
Main Menu

Products

  • Monoclonal Antibodies
  • Polyclonal Antibodies
  • Assay Kits
  • Proteins
  • Lab Supplies

Applications

  • G Protein | GTPase
  • Oncogene
  • cAMP/cGMP

Technical Supports

  • Company News
  • Published Pictures from Citations

Contact

中国武汉办事处

  • (+86) 027-63497286
  • [email protected]
  • 湖北省武汉市东湖高新区高新大道 666 号光谷生物城 B1 栋三楼

Copyright © 2010-2024 NewEast Biosciences | All rights reserved

GTPase & Oncogene | cGMP & cAMP | Nanobody | Bioactive Transmembrane Proteins | Circulating Tumor Cells | Lipoproteins

Từ khóa » K117n