Thi Ngoc Nhu Phan | University Of Gothenburg

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4. Thi Ngoc Nhu Phan

Thi Ngoc Nhu Phan

Senior Lecturer

Department of Chemistry & Molecular Biology Telephone +46 720-35 20 02 Mail [email protected] Visiting address Medicinaregatan 7 B 41390 Göteborg Postal address Box 462 40530 Göteborg

About Thi Ngoc Nhu Phan

The research within the Phan group is focused on the development and application of mass spectrometry imaging (ToF-SIMS, NanoSIMS, MALDI), super-resolution stimulated emission depletion (STED) microscopy, electron microscopy, and correlative imaging using combinations of these modalities to understand the structural and functional relationship of biomolecules in neural stem cells, neuronal cells and in the brain. Another research topic is the development of novel labelling probes for correlative imaging. The probes enable imaging multiple targeted molecules at micro and nanoscale resolution using the combination of SIMS, fluorescence microscopy, and electron microscopy.

The developed technologies have been applied to the following biological questions:

• Organization of structural lipids at synaptic membrane, and its relation to neuronal activity.

• Targeted molecular imaging of the brain, structural and functional relation in brain activity.

• Molecular turnover in neuronal cells and brain, and correlation to neuro-degeneration.

• Molecular turnover, dynamics and regulation in neural stem cells.

• Stress granule pathway and molecular transition in neuronal cells.

LINKS

Nhu Phan group home page: http://thenhuphangroup.com/

Google Scholar

TEACHING

KEM070 - Basic Analytical Chemistry

KEM082 - Advanced Analytical Chemistry

KEA310 - Introduction to Analytical Chemistry

Publications

• Computer Vision-Assisted Data Analysis for Correlative Electron Microscopy and Secondary Ion Mass Spectrometry Imaging

  Andre Du Toit, Alicia Andrea Lork, Carl Ernst, Thi Ngoc Nhu Phan ANALYTICAL CHEMISTRY - 2025

• Lipid organization and turnover in the plasma membrane of human differentiating neural progenitor cells revealed by time-of-flight secondary ion mass spectrometry imaging

  Emmanuel Berlin, Alicia Lork, Martin Bornecrantz, Carl Ernst, Nhu TN Phan TALANTA - 2024

• Subcellular protein turnover in human neural progenitor cells revealed by correlative electron microscopy and nanoscale secondary ion mass spectrometry imaging

  Alicia Lork, Stefania Rabasco, Carl Ernst, Andre Du Toit, Silvio O. Rizzoli, Nhu TN Phan CHEMICAL SCIENCE - 2024

• Characterization of Stress Granule Protein Turnover in Neuronal Progenitor Cells Using Correlative STED and NanoSIMS Imaging

  Stefania Rabasco, Alicia Lork, Emmanuel Berlin, Tho D. K. Nguyen, Carl Ernst, Nicolas Locker, Andrew G Ewing, Nhu TN Phan International Journal of Molecular Sciences - 2023

• Single Exosome Amperometric Measurements Reveal Encapsulation of Chemical Messengers for Intercellular Communication

  K. K. Hu, Kim Vo, F. Wang, X. Zhang, Chaoyi Gu, N. Fang, Nhu TN Phan, Andrew G Ewing Journal of the American Chemical Society - 2023

• White matter integrity in mice requires continuous myelin synthesis at the inner tongue

  Martin Meschkat, Anna M. Steyer, Marie-Theres Weil, Kathrin Kusch, Olaf Jahn, Lars Piepkorn, Paola Aguei-Gonzalez, Nhu TN Phan, Torben Ruhwedel, Boguslawa Sadowski, Silvio O. Rizzoli, Hauke B. Werner, Hannelore Ehrenreich, Klaus-Armin Nave, Wiebke Moebius NATURE COMMUNICATIONS - 2022

• Visualization of Partial Exocytotic Content Release and Chemical Transport into Nanovesicles in Cells

  Tho D. K. Nguyen, Lisa J. Mellander, Alicia Lork, Aurélien Thomen, M. Philipsen, M. E. Kurczy, Nhu TN Phan, Andrew G Ewing Acs Nano - 2022

• Quantitative Chemical Imaging at the Cellular Level: SIMS, Fluorescence, and Correlative Techniques

  Tho D. K. Nguyen, Alicia Lork, Andrew G Ewing, Nhu TN Phan Single Cell ‘Omics of Neuronal Cells - 2022

• Chemical Imaging and Analysis of Single Nerve Cells by Secondary Ion Mass Spectrometry Imaging and Cellular Electrochemistry

  A. A. Lork, K. L. L. Vo, Nhu TN Phan Frontiers in Synaptic Neuroscience - 2022

• Localization and absolute quantification of dopamine in discrete intravesicular compartments using nanoSIMS imaging

  Stefania Rabasco, Tho D. K. Nguyen, Chaoyi Gu, Michael E. Kurczy, Nhu TN Phan, Andrew G Ewing International Journal of Molecular Sciences - 2022

• Dynamic Visualization and Quantification of Single Vesicle Opening and Content by Coupling Vesicle Impact Electrochemical Cytometry with Confocal Microscopy.

  Ying-Ning Zheng, Tho D. K. Nguyen, Johan Dunevall, Nhu TN Phan, Andrew G Ewing ACS measurement science Au - 2021

• Electrochemical Measurements Reveal Reactive Oxygen Species in Stress Granules**

  Keke Hu, E. Relton, N. Locker, Nhu TN Phan, Andrew G Ewing Angewandte Chemie - International Edition - 2021

• Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging

  Felix Lange, Paola Agüi-Gonzalez, Dietmar Riedel, Nhu TN Phan, Stefan Jakobs, Silvio O. Rizzoli PLoS ONE - 2021

• Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry

  P. Agui-Gonzalez, T. M. Dankovich, S. O. Rizzoli, Nhu TN Phan Nanomaterials - 2021

• Secondary Ion Mass Spectrometry Imaging Reveals Changes in the Lipid Structure of the Plasma Membranes of Hippocampal Neurons following Drugs Affecting Neuronal Activity

  P. Aguei-Gonzalez, G. B. Bao, M. A. G. de Castro, S. O. Rizzoli, Nhu TN Phan Acs Chemical Neuroscience - 2021

• Mass Spectrometry Imaging of Cocaine, Drug Removal and Methylphenidate AlterPhospholipids in Drosophila Brain

  Mai H. Philipsen, Nhu TN Phan, John S. Fletcher, Andrew G Ewing Biorxiv preprint - 2020

• Interplay between Cocaine, Drug Removal, and Methylphenidate Reversal on Phospholipid Alterations in Drosophila Brain Determined by Imaging Mass Spectrometry

  Mai H. Philipsen, Nhu TN Phan, John S. Fletcher, Andrew G Ewing ACS Chemical Neuroscience - 2020

• Subcellular Mass Spectrometry Imaging and Absolute Quantitative Analysis across Organelles

  Aurélien Thomen, Neda Najafinobar, Florent Penen, Emma Kay, Pratik P. Upadhyay, Xianchan Li, Nhu TN Phan, Per Malmberg, Magnus Klarqvist, Shalini Andersson, Michael E. Kurczy, Andrew G Ewing ACS nano - 2020

• High-Resolution Molecular Imaging and Its Applications in Brain and Synapses

  Nhu TN Phan, S. O. Rizzoli Volume Microscopy - 2020

• Chemical Analysis of Single Cells

  Pieter E. Oomen, Mohaddeseh A. Aref, Ibrahim Kaya, Nhu TN Phan, Andrew G Ewing Analytical Chemistry - 2019

• SIMS imaging in neurobiology and cell biology

  P Agüi-Gonzalez, S Jähne, Nhu TN Phan Journal of Analytical Atomic Spectrometry - 2019

• Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins

  K N Richter, C Patzelt, Nhu TN Phan, S O Rizzoli Scientific Reports - 2019

• Boron-Containing Probes for Non-optical High-Resolution Imaging of Biological Samples

  S Kabatas, P Agüi-Gonzalez, K Ann Saal, S Jähne, F Opazo, S O Rizzoli, Nhu TN Phan Angewandte Chemie - International Edition - 2019

• Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry

  S Kabatas, P Agüi-Gonzalez, R Hinrichs, S Jähne, F Opazo, U Diederichsen, S O Rizzoli, Nhu TN Phan Journal of Analytical Atomic Spectrometry - 2019

• Mass Spectrometry Imaging Shows Cocaine and Methylphenidate Have Opposite Effects on Major Lipids in Drosophila Brain

  Mai H. Philipsen, Nhu TN Phan, John S. Fletcher, Per Malmberg, Andrew G Ewing Acs Chemical Neuroscience - 2018

• Measuring synaptic vesicles using cellular electrochemistry and nanoscale molecular imaging

  Nhu TN Phan, Xianchan Li, Andrew G Ewing Nature Reviews Chemistry - 2017

• Laser Desorption Ionization Mass Spectrometry Imaging of Drosophila Brain Using Matrix Sublimation versus Modification with Nanoparticles

  Nhu TN Phan, Amir Saeid Mohammadi, Masoumeh Dowlatshahi Pour, Andrew G Ewing Analytical Chemistry - 2016

• Intact lipid imaging of mouse brain samples: MALDI, nanoparticle-laser desorption ionization, and 40 keV argon cluster secondary ion mass spectrometry

  Amir Saeid Mohammadi, Nhu TN Phan, John S. Fletcher, Andrew G Ewing Analytical and Bioanalytical Chemistry - 2016

• Lipid Structural Effects of Oral Administration of Methylphenidate in Drosophila Brain by Secondary Ion Mass Spectrometry Imaging

  Nhu TN Phan, John S. Fletcher, Andrew G Ewing Analytical Chemistry - 2015

• Mass spectrometry based analysis of drugs, neurotransmitters and lipids in invertebrate model systems

  Nhu TN Phan 2015

• Imaging Mass Spectrometry for Single-Cell Analysis

  Nhu TN Phan, John S. Fletcher, Andrew G Ewing Reference Module in Chemistry, Molecular Sciences and Chemical Engineering - 2014

• ToF-SIMS imaging of lipids and lipid related compounds in Drosophila brain

  Nhu TN Phan, John S. Fletcher, Peter Sjövall, Andrew G Ewing Surface and Interface Analysis: 19th International Conference on Secondary Ion Mass Spectrometry (SIMS) - 2014

• Mass spectrometry imaging of freeze-dried membrane phospholipids of dividing Tetrahymena pyriformis

  Ingela Lanekoff, Nhu TN Phan, C. T. Van Bell, N. Winograd, Peter Sjövall, Andrew G Ewing Surface and Interface Analysis - 2013

• Capillary Electrophoresis-Mass Spectrometry-Based Detection of Drugs and Neurotransmitters in Drosophila Brain

  Nhu TN Phan, Jörg Hanrieder, Carina Berglund, Andrew G Ewing Analytical Chemistry - 2013

• Imaging mass spectrometry in neuroscience.

  Jörg Hanrieder, Nhu TN Phan, Michael E. Kurczy, Andrew G Ewing ACS chemical neuroscience - 2013

• Oral administration of methylphenidate blocks the effect of cocaine on uptake at the Drosophila dopamine transporter.

  E Carina Berglund, Monique A Makos, Jacqueline Keighron, Nhu TN Phan, Michael L Heien, Andrew G Ewing ACS chemical neuroscience - 2013